However, their extreme slow growth rate has impeded its application for renewable. The performance of supercritical co2 extraction of lipids from botryococcus braunii for biodiesel production was studied. The green planktonic microalga, botryococcus braunii is well known for its ability to produce high amounts of hydrocarbon and has already been proposed as a renewable energy source. Pdf isolation and characterization of hydrocarbon producing. Outdoor open pond batch production of green microalga.
Influence of co2 on growth and hydrocarbon production in. This study investigated the effects of exogenous plant growth regulators pgrs 6benzylaminopurine 6ba, abscisic acid aba, 2,4epibrassinolide ebr, ethephon eth, gibberellic. Theeffectofnutrientsandenvironmentalconditionsonbiomass. Mar 14, 2020 botryococcus braunii, a green alga that can produce a variety of secondary metabolites such as lipids, protein, and carotenoid, is considered a potential biodiesel feedstock for industrial commercialization. Direct transesterification of microalga botryococcus braunii. Pdf cultivation of botryococcus braunii strain in relation of its use. Characteristics of microalgal biofuel from botryococcus braunii. Effect of critical parameters on biomass yield from. A new mechanism for efficient hydrocarbon electroextraction from. Cultivation of botryococcus braunii strain in relation of its use for biodiesel production article pdf available january 20 with 1,553 reads how we measure reads. Evaluation of media and light source effects on the growth of.
Cultivation of botryococcus braunii strain in relation of. The colonial microalga botryococcus braunii has been brought to peoples attention for its conspicuous ability to accumulate a variety of lipids including hydrocarbons. Methodology algal culture and biomass preparation in present study, microalga botryococcus braunii was. Colonies held together by a lipid biofilm matrix can be found in temperate or tropical oligotrophic lakes and estuaries, and will bloom when in the presence of elevated levels of dissolved inorganic phosphorus. Until now, no studies have been performed on the effects of light quality on b. The practice of farming cultivating is known as algae culture. Influence of co2 on growth and hydrocarbon production in botryococcus braunii. A new mechanism for efficient hydrocarbon electroextraction. Botryococcus b raunii oils was done in relation to their suitability for transesterification i. Botryococcus braunii has a cell nucleus with a size. Botryococcus braunii bb is a green, pyramidshaped planktonic microalga that is of potential importance in the field of biotechnology. The botryococcus braunii strain was studied by using two media bbm and 3nbbm and its potential for biodiesel production was established.
This study focuses on extracting oil from the microalgae matrix rather than the cells, eliminating the need for an excessive electric field to. Biodiesel biodiesel is an alternative fuel similar to conventional or fossil diesel. Hydrocarbon production from secondarily treated piggery wastewater by the green alga botryococcus braunii jin young an, sang jun sim, jin suk lee, byung woo kim department of chemical and biological engineering. Co2 removal from industrial flue gas using botryococcus. Direct transesterification of microalga botryococcus. Effects of stress conditions on lipid production by. For instance, botryococcus braunii race a strain ccap 8072 was previously assessed to be of potential interest for biofuel production, because. Specimens were isolated, cultured and examined for its hydrocarbon content, morphological features and dna structural resemblance with the known strain to confirm its identity.
Molecular and biochemical characterization of hydrocarbon. Pdf cultivation of botryococcus braunii strain in relation. Uvb mutations were carried out to see the effect in microalgae growth at various phs 38. Botryococcus braunii an overview sciencedirect topics. Genome sequence of fuelproducing alga botryococcus braunii. Oct 17, 2017 production of biodiesel from botryococcus braunii 2. So far, however, there has been little discussion about microalgae. The quite common oil content by weight of dry biomass present in. The effects of stress conditions on neutral lipid accumulation by the microalgae botryococcus braunii, tetraselmis suecica and coccomyxa were investigated to assess their suitability for biodiesel production. Katya velichkova department of biology and aquaculture, faculty of agriculture, trakia university, students campus, 6000 stara zagora, bulgaria. Acoi 58 and acoi 1257, two different portuguese strains of colonial green microalga botryococcus bruanii kutz obtained from coimbra collection of algae acoi, were evaluated on their potential for biofuel production, which was assessed from batch culture in photobioreactors under indoors and outdoors conditions using pretreated waste water and semisynthetic culture media. Detection of the oilproducing microalga botryococcus braunii. Thus, marine macroalgae serve as a potential renewable raw material for biodiesel production. Nov 18, 2019 the green microalga botryococcus braunii produces hydrocarbon oils at 2575% of its dry weight and is a promising source of biofuel feedstock.
Aug 28, 2015 the colonial microalga botryococcus braunii has been brought to peoples attention for its conspicuous ability to accumulate a variety of lipids including hydrocarbons. Plant growth regulators affect biomass, protein, carotenoid. Evaluation of two portuguese strains of botryococcus braunii. Theeffectofnutrientsandenvironmentalconditionsonbiomass and. Botryococcus braunii are unicellular, planktonic, ovalshaped microalgae. The green alga botryococcus braunii shows great potential for biodiesel production due to its ability to produce high amounts of hydrocarbon. Biodiesel is an alternative fuel source that is obtained from renewable biomass, such as canola, soybean, corn, palm, sunflower, and algae. Botryococcus braunii, microalgae, bubble column photo bioreactor, biofuels. Sep 29, 2008 botryococcus braunii, a green colonial microalga, is an unusually rich renewable source of hydrocarbons and other chemicals. Jan 04, 2018 botryococcus braunii bb is a green, pyramidshaped planktonic microalga that is of potential importance in the field of biotechnology. There are several reports on the implementation of botryococcus in waste water treatment from different countries. It is reported that 86% of th e dry weight of botryococcus braunii can be long chain hydrocarbons 15. The experiments were conducted at 5080c and 200250 bar.
Microalgae botryococcus braunii is a potential biodiesel producer as an alternative for fossil fuels due to its high lipid content. This stu dy addresses this by examining the effects of three growth media on biomass and oil production in two b. Botryococcus braunii is a green, colonial microalga that can produce up to 75 % of its dry weight as liquid hydrocarbons that can be converted to a sustainable biofuel. Study of uvb mutation effect on ph resistance and lipid. Botryococcus biomass production hamper its commercial exploitation. Ravishankara bioresource technology 98 2007 560564 pdf.
High biofuel production of botryococcus braunii using optimized cultivation strategies. The individuals live in a biofilm community that helps contribute to their external hydrocarbon production 3. The green algae botryococcus braunii is widely recognized as a source of oil, including hydrocarbons. Faculty of agriculture, trakia university, stara zagora, bulgaria. In line with this, an experiment was conducted to determine the growth and lipid production in two microalgal species botryococcus braunii and fragilaria brevistriata by nutrient limitation experiment using different concentrations of nitratenitrogen 20, 10, 1. Botryococcus braunii for biodiesel production sciencedirect. Thus this investigation was undertaken to grow the cultures of botryococcus braunii in photobioreactor and for the biofuels production. A vital element of the economic production of algae biofuels is growing the algae efficiently. Methodology algal culture and biomass preparation in present study, microalga botryococcus braunii was used for the experiment. The effects of light quality on the morphology and. Transesterification cannot be used to make biodiesel from botryococcus oils. Tim devarenne, agrilife research biochemist and principal investigator in college station. This study examines the effects of nutrients nitrogen and iron and environmental conditions temperature, light intensity and photoperiod on biomass and oil production in two b.
Three forms of these algae colony have developed diverse metabolic tactics to produce the alage oil 7,8. Currently, biodiesel is generally derived from plant crops, but a more. Comparative analysis of growth and lipid production in two. Botryococcus braunii, a green colonial microalga, is an unusually rich renewable source of hydrocarbons and other chemicals. Hydrocarbon production from secondarily treated piggery. Summarizing the nature of this alga is fundamental to establish the detail environment conditions for optimal biomass growth and the production of lipids, as well as upstream processing technologies for biodiesel. Production of biofuel by using micro algae botryococcus. Production of biodiesel from botryococcus braunii 2. Botryococcus braunii is a single green cell microalgae and is commonly found in the lakes, ponds, or brackish waters and the sea with a chlorophyll content of 1.
Growth of botryococcus braunii race a and production of its constituents viz, hydrocarbon, carbohydrate, fatty acid, and carotenoids were inxuenced by diverent levels of salinity. This is because these oils are not vegetable oils in the common meaning, in which they are. Previously, botryococcus braunii was cultivated in piggery wastewater for hydrocarbon production. The green microalga botryococcus braunii produces hydrocarbon oils at 2575% of its dry weight and is a promising source of biofuel feedstock.
Botryococcus braunii, a green alga that can produce a variety of secondary metabolites such as lipids, protein, and carotenoid, is considered a potential biodiesel feedstock for industrial commercialization. Botryococcus braunii is a microalga with an extracellular hydrocarbon. Botryococcus braunii kutzing, 1849 is a planktonic, widespread, colonial microalga and a member of the chlorophyceae. One type of microalgae that can be utilized to biodiesel is botryococcus braunii. Some microalgae species can produce ten times more oil than the typical oil crops 1. Paper open access fatty acids from microalgae botryococcus. Microalgae are reported as the potential resources to produce lipid from their biomass cell. Biodiesel fuels, derived from a variety of animal, plant and microbial feedstocks are promising renewable alternative diesel fuels in view of energy. Botryococcus braunii as a potential candidate for the waste.
The effect of media on biomass and oil production in. The lipids are present in form of triacylglycerides tags that are utilized for biodiesel production. Investigating the effects of media and light sources on the. Botryococcus braunii has great potential for alga culture. Hydrocarbons can constitute up to 75% of the dry mass of b. The vast majority of these hydrocarbons are botryocuccus oils.
Botryococcus braunii chlorophyta, botryococcaceae is a colonyforming green microalga that produces large amounts of liquid hydrocarbons, which can be converted into transportation fuels. A and b races are known to accumulate high level of lipids. Botryococcus braunii for biodiesel production request pdf. Reduction of nitrogen levels was carried out to see the effect on the growth and lipid production of microalgae. Transcriptomic analysis of a moderately growing subisolate.
Botryococcus is often described as relatively difficult to grow. Summarizing the nature of this alga is fundamental to establish the detail environment conditions for optimal biomass growth and the production of lipids, as well as. Botryococcus braunii is characterised by an apparent ability to. Colonies held together by a lipid biofilm matrix can be found in tropical gold or oligotrophic lakes and estuaries, and will bloom when in the presence of elevated levels of inorganic dissolved phosphorus. A dissertation submitted in partial satisfaction of the requirements for the.
Botryococcus braunii is a microalga with an extracellular hydrocarbon matrix, which makes it an appropriate green energy source. Cultivation of botryococcus braunii strain in relation of its use for biodiesel production abstract authors address. Studies on batch and continuous cultures of botryococcus. Investigating the effects of growth medium and light sources.
Cultivation of botryococcus braunii strain in relation of its. Recent understanding that specific algae have high hydrocarbon production potential has attracted considerable attention. At the same time the micro alga botryococcus can alternatively serve as a biological source for the production of biodiesel. Algae is known as one of the most promising sources for biodiesel production. The extracellular matrix is a complex, three component system that all works together to produce the external hydrocarbons as well as protects each cell 14. Production of biofuel by using micro algae botryococcus braunii.
Produced from straight vegetable oil animal fats waste cooking oil. Samples of green colonial unicellular microalga botryococcus braunii were collected from bear shola falls at kodaikanal latitude 10. Effect of salinity on growth of green alga botryococcus braunii and its constituents a. Botryococcus braunii is a green, pyramidshaped planktonic microalga that is of potentially great importance in the field of biotechnology. Aug 22, 2018 biodiesel is an alternative fuel source that is obtained from renewable biomass, such as canola, soybean, corn, palm, sunflower, and algae. Evect of salinity on growth of green alga botryococcus.
1037 1279 1492 1187 245 12 753 1373 1231 581 747 488 131 167 1583 1500 1527 1089 1482 1400 953 491 529 577 1495 23 655 405 940